The group calls on the front-runner of the Republicans to clarify his stance amidst the broader claims from opponents of Trump that his many positions on key issues can be difficult to determine.
The video, which the Log Cabin Republicans released, uses past remarks made by Trump to challenge his recent statements made against gay marriage.
In the Republican debate last week, for example, Trump called the decision by the U.S. Supreme Court that legalized gay marriage, a wrong and illegitimate decision.
The video asks the question, where does Mr. Trump really stand when it comes to marriage equality, before showing a number of headlines as well as comments suggesting his support for gay marriage.
The decision was made and that is the law of the United States, said Trump in during one interview as he referred to the case in the Supreme Court of Obergefell v. Hodges that in 2015 legalized gay marriage.
In another clip in the video, Trump can be seen saying that it is a conversation that more and more are having and that anything could happen.
The video then finishes as Trump is saying that people can trust him on traditional marriage.
This video is the most recent in a series of attacks against Trump by rivals in 2016 as well as conservative activists that have accused the Republican front-runner of lacking the credentials to be a conservative, claiming had made changes to his opinions and positions on many issues over the past years.
Trump however has steadfastly maintained that any leader who is successful must also be flexible as well as open to reconsidering certain policy positions, just as he has had to do.
In separate news, police were searching on Monday in Idaho for a gunman who shot a pastor on Sunday outside his church. The shooting came just a day after the pastor had led a prayer during a rally for Ted Cruz a Republican presidential nominee.
The pastor, Tim Remington, was found outside his church with a number of gunshot wounds. His nephew and other members of the church said that Remington had been shot in the back four times while walking to his vehicle.